oc cell line skov3  (Procell Inc)

Journal: Experimental and Therapeutic Medicine

Article Title: Long non-coding RNA KCNQ1OT1 promotes ovarian cancer cell malignant characteristics by targeting the miR-140-5p/KLK10 axis

doi: 10.3892/etm.2026.13129

Figure Lengend Snippet: Expression of target genes in OC datasets and cell lines. Principal component analysis plots showing expression patterns in the (A) GSE66957 and (C) GSE47841 datasets. Volcano plots illustrating differentially expressed genes in the (B) GSE66957 and (D) GSE47841 datasets. Differential expression of (E) KCNQ1OT1 ( GSE66957 ), (F) KLK10 ( GSE66957 ) and (G) miR-140-5p ( GSE47841 ). (H) Relative expression levels of KCNQ1OT1, KLK10 and miR-140-5p in SKOV3 OC cells and IOSE80 normal ovarian epithelial cells. * P<0.05 and ** P<0.01. OC, ovarian cancer; KCNQ1OT1, potassium voltage-gated channel subfamily Q member 1 opposite strand/antisense transcript 1; KLK10, kallikrein-related peptidase 10; miR, microRNA; Dim, dimension; FC, fold change.

Article Snippet: IOSE80 (normal ovarian epithelial) and SKOV3 (OC) cell lines were sourced from Procell Life Science & Technology Co., Ltd. IOSE80 cells were maintained in DMEM (Gibco; Thermo Fisher Scientific, Inc.) and SKOV3 cells in McCoy's 5A medium (Gibco; Thermo Fisher Scientific, Inc.), both supplemented with 10% FBS (HyClone; Cytiva) and 1% penicillin-streptomycin, at 37 ̊C in a 5% CO 2 humidified incubator.

Techniques: Expressing, Quantitative Proteomics

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A – D CCK8 assay to assess the effect of UHRF1-knockdown and UHRF1-overexpressed OC cell lines on the proliferation. E EDU cell proliferation assay showing the effect of UHRF1-knockdown OC cell lines. F Colony formation assay showing the effect of UHRF1-knockdown OC cell lines on colony-forming ability. G , H Transwell migration and invasion assays showing the effect of UHRF1-knockdown OC cell lines on migration and invasion abilities. I Wound healing assay showing UHRF1-knockdown SKOV3 cell lines migration. J Tumorigenicity assay in nude mice showing the effect of UHRF1-knockdown SKOV3 cell lines on tumor formation. K Ki67 staining of tumor tissues to assess the effect of UHRF1 knockdown on cell proliferation. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: CCK-8 Assay, Knockdown, Proliferation Assay, Colony Assay, Migration, Wound Healing Assay, Tumorigenicity Assay, Staining

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A Volcano plot showing differential expression analysis between sh-NC SKOV3 cells and sh-UHRF1#2 SKOV3 cells, highlighting key potential downstream molecules. B Heatmap displaying the expression of HIF-1 signaling pathway genes between the knockdown and control groups. C , D KEGG pathway enrichment analysis of differentially expressed genes (DEGs), bubble chart shows the enriched pathways; circle plot displays the specific genes within the pathways. E GO-MF enrichment analysis of DEGs, the specific pathway names can be found in (Supplementary Table ). F GSEA enrichment analysis of DEGs. G Correlation analysis of UHRF1 and HIF-1 signaling pathway genes using GEPIA2.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Quantitative Proteomics, Expressing, Knockdown, Control

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A Tissue immunofluorescence (IF) confirmed the colocalization of UHRF1 and HIF-1α in tissues. B Silver staining of mass spectrometry samples. C Co-immunoprecipitation (co-IP) confirmed the interaction between UHRF1 and HIF-1α in SKOV3 cells. D Forward and reverse co-IP validated the interaction between UHRF1 and HIF-1α in 293T cells. E IB analysis of interaction domains between HIF-1α and UHRF1 using truncated plasmids co-transfected in 293T cells. F Truncated sequence map of UHRF1. G Truncated sequence map of HIF-1α. H WB analysis of interaction domains between UHRF1 and HIF-1α using truncated plasmids co-transfected in 293T cells. I WB analysis of UHRF1’s effect on the nuclear translocation of HIF-1α. J IF analysis of UHRF1’s effect on HIF-1α nuclear translocation.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Immunofluorescence, Silver Staining, Mass Spectrometry, Immunoprecipitation, Co-Immunoprecipitation Assay, Transfection, Sequencing, Translocation Assay

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A UMAP plot with clusters demarcated by colors demonstrating 12 distinct clusters based on gene expression differences for epithelial cells passing quality control. B The UMAP plot demarcated by colors showing the two groups of OC tumors (malignant) and nonmalignant ovarian tissues. C Expression of HIF signaling pathway genes in tumor and normal tissues. D Expression of HIF signaling pathway genes in each epithelial cell cluster. E UMAP plot color coded for the expression (blue to purple) of marker genes for HIF signaling pathway genes. F The proportion of epithelial cell types relative to the total epithelial cell count in each clinical patient. G KEGG pathway enrichment analysis of epithelial-cluster6 genes. H , I UHRF1-knockout and UHRF1-overexpressed SKOV3 cell lines were treated with CoCl₂ for 24 h, and WB analysis was performed to assess the expression levels of glycolysis-related proteins. J – M UHRF1-knockdown and UHRF1-overexpressed OC cell lines were cultured under normoxic and hypoxic conditions for 48 h, and glucose consumption and lactate production were measured. Data are presented as mean ± SD from three independent experiments. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Gene Expression, Control, Expressing, Marker, Cell Counting, Knock-Out, Knockdown, Cell Culture

Journal: Cell Death & Disease

Article Title: UHRF1-mediated HIF-1α stabilization promotes ovarian cancer through metabolic reprogramming and angiogenesis

doi: 10.1038/s41419-025-08033-w

Figure Lengend Snippet: A , B Periodic acid-Schiff (PAS) (pink) and CD31 (brown) double staining of tumor tissues from xenograft mice. PAS+/CD31+ tubular structures represent conventional blood vessels lined with CD31-positive endothelial cells. Adjacent PAS+/CD31− tubular structures suggest vasculogenic mimicry (VM). C , D WB analysis of VEGF expression levels in cells treated with CoCl₂ for 24 h. E Representative images of the tube formation assay in SKOV3 stable cell lines. F Representative images of the tube formation assay in HUVEC cells after 72 h of culture with conditioned medium from the stable SKOV3 cell line. G Images of the Transwell migration assay and tube formation assay in HUVEC cells after 72 hours of culture with conditioned medium from the stable SKOV3 cell line. H , I Images of the Transwell migration assay and tube formation assay in HUVEC cells after 72 h of culture with conditioned medium from the stable SKOV3 cell line, followed by treatment with the HIF-1α inhibitor PX-478. J , K IC50 analysis of Bevacizumab in UHRF1-knockdown A2780 cell lines. ** p < 0.01; *** p < 0.001; **** p < 0.0001.

Article Snippet: The human OC cell line SKOV3 (CL-0215) was purchased from Wuhan Procell Life Science & Technology Co., Ltd., and A2780 (STCC10706P-1) was purchased from Wuhan Servicebio Technology Co., Ltd.

Techniques: Double Staining, Expressing, Tube Formation Assay, Stable Transfection, Transwell Migration Assay, Knockdown